Two sensitive spectrophotometric methods; using first derivative 1D spectrophotometry (method I) and dual wavelength spectrophotometry (method II), were developed and validated for the simultaneous determination of dexketoprofen trometamol and tramadol HCl in binary mixtures and synthetic mixture. Method I was based on measuring 1D at 242 nm for determination of dexketoprofen trometamol and 1D at 232 nm for determination of tramadol HCl. Method II was based on measuring the absorbance difference between 259 nm and 281.6 nm for determination of dexketoprofen trometamol and between 225 nm and 281 nm for determination of tramadol HCl. Linearity range for dexketoprofen trometamol using methods I and II was found to be 4.00–30.00 µg.ml-1. For tramadol HCl the linearity range was found to be 20.00–100.00 µg.ml-1 for both methods. The developed methods were successfully applied for the determination of dexketoprofen trometamol and tramadol HCl in synthetic mixture containing all possible excipients present in tablet dosage form. The mean percentage recovery values were found to be 98.68±0.34 and 99.34±0.48 for method I and 101.05±0.41, 101.13±0.54 for method II for dexketoprofen trometamol and tramadol HCl, respectively.
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